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Factors Influencing Venous Remodeling in the Development of Varicose Veins of the Lower Limbs - PMC

[DOI] [PubMed] [Google Scholar] Penedo MARivera-Baltanás TPérez-Rodríguez DAllen JBorrajo AAlonso-Crespo DFernández-Pereira CNieto-Araujo MRamos-García SBarreiro-Villar C, et al

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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 °C in a humidified incubator in the presence of 5% CO 2 for 24 h–72 h