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PreScission Cleavage of the GST Centrifuge the GST beads, remove the supernatant and wash the GST beads with P5 buffer (50 mM NaHPO 4 pH 7.0, 500 mM NaCl, 10% glycerol, 0.05% Triton-X-100, 5 mM imidazole)

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References 1.Kimura R, Murata T