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Lysates were then sonicated, boiled, and stored at −20 °C until needed

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For example, target cells infected with primary HIV encoding functional Nef and Vpu accessory proteins, which downregulate CD4 expression on the surface of infected cells to evade host defences, are largely resistant to ADCC mediated by a class of antibodies specific for the CD4-induced epitope (CD4i) of gp120