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Here we determined the mechanisms and rate constants for the glutathione/glutaredoxin-dependent redox reactions of the widely used redox probe roGFP2 and addressed the question of how mono- and dithiol glutaredoxins catalyze the reduction of roGFP2(S 2 ) as a model non-glutathione protein disulfide substrate

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Materials and methods Reagents EMD1214063 (c-MET tyrosine kinase inhibitor) was developed by Merck (Darmstadt, Germany) 32