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For example, the probiotic strain Limosilactobacillus reuteri D8 activated the Wnt/β-catenin signaling pathway, increasing the number of Lgr5 + and lysozyme + cells in the crypts, and stimulating epithelial proliferation both in the murine intestine and in organoid cultures ( Akkermansia muciniphila administration accelerated Lgr5 + stem cell proliferation and enhanced Paneth and goblet cell differentiation in the small intestine, effects mediated via GPR41/43 receptor activation ( Limosilactobacillus reuteri ATCC PTA 4659 restored the expression of heat shock proteins Hsp70 and Hsp25 in the murine intestinal epithelium, improved tight junction structure, and reduced inflammatory responses, thereby strengthening the mucosal barrier and lowering systemic inflammation ( 9 Colony-forming unit (CFU) of the probiotic Lactobacillus plantarum HAC01 exerted strong antidiabetic activity in a high-fat diet (HFD) mouse model, comparable to metformin

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