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During the first 28 days post-opening: Benzyl alcohol concentration remains effective at preventing bacterial growth The preservative provides reliable protection against typical environmental microbial exposure during normal vial access Any microbial contamination introduced during typical research handling is suppressed Past 28 days post-opening: Benzyl alcohol effectiveness decreases through evaporation, oxidation, and other chemical changes The preservative's ability to suppress new microbial introductions decreases Risk of contamination compromising subsequent reconstitutions increases The 28-day window also aligns conveniently with the typical reconstituted peptide shelf life — a researcher who reconstitutes a peptide on the same day they open a fresh bacteriostatic water vial will typically finish both at roughly the same time