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Regulatory role in quality control processes The ubiquitin-proteasome system and autophagy are regulated by taurine as a part of its role in regulating quality control processes
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Both urea and guanidinium chloride are powerful denaturants and would unfold the protein, if the protein binds to the resin in presence of these denaturants, it can be said with certainty that folded protein hides the his-tag and prevents its binding to the IMAC resin
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