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Npas4 is a transcription factor with pronounced expression in the brain, including the hippocampus, where it plays a pivotal role in regulating the formation and maintenance of inhibitory synapses in response to excitatory synaptic activity ( Npas4 may act as a neuroprotective factor in the context of PM, where the influx of excitatory amino acids into the CNS can induce neuronal excitotoxicity ( Npas4 , as further validated by RT-qPCR ( Figure 3 ), cannot be attributed to an increase in its promoter methylation induced by B12